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KMID : 0613820110210030400
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Journal of Life Science
2011 Volume.21 No. 3 p.400 ~ p.405
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The Autophagic Response to Exercise Training of the Skeletal Muscle Fibers in Young and Old Mice
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Kim Yong-An
Kim Young-Sang
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Abstract
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Autophagy, a highly conserved mechanism of internal quality control, is essential for the maintenance of cellular homeostasis and for the orchestration of an efficient cellular response to stress. During aging, the efficiency of autophagic degradation declines and intracellular waste products accumulate. Therefore, the aim of this study is to investigate the effects of exercise on autophagic response in skeletal muscle. Twenty-four Young (4 month) and Old (12 month) ICR-type white male mice were divided into a control group (CON: n=6) and exercise training group (Tr: n=6) after an adaptation period of 1 week. Exercise consisted of treadmill running at 16.4 m/min with a 4% incline, 40 min/day and 5 days/week for 8 weeks. Cervical dislocation was performed at 48 hours after the last round of exercise, after which the gastrocnemius skeletal muscle were immediately collected. The results of verifying autophagy formation showed that the Sarcopenia index was decreased in the Old mice compared to the Young. However, it increased with exercise training in the Old. Lipidation LC3-Ⅱ, Becline-1, and Atg7 were decreased in the Old mice compared to the Young. However, Lipidation LC3-Ⅱ was significantly increased in the trained Old mice (Young:1 Vs Old:1.32±0.042, p＜0.05). Based on these data, we suggest that autophagy regulatory events are the attenuated in Old mice, but that they are enhanced with exercise training.
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KEYWORD
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Aging, Skeletal muscle, Exercise Training, Autophagy
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